Page 204 - Livre électronique des RFTP 2023

P141.WHAT MOLECULAR CHANGES OCCUR DURING
PULMONARY AGING IN INDIVIDUALS WITH CHRONIC

OBSTRUCTIVE PULMONARY DISEASE (COPD)?

A.SAYHI1,2, Y.ABDELHEDI1, A.YAHYAOUI1, K.MAHMOUDI1, M. ABDESSALEM1, N.LAZREG1,
F.GUEZGUEZ1,2, I.GHANNOUCHI1,2, S.ROUATBI1,2

1LABORATORY OF PHYSIOLOGY AND FUNCTIONAL EXPLORATIONS, FARHAT HACHED HOSPITAL,
SOUSSE 2RESEARCH LABORATORY, LR12SP09

INTRODUCTION : The lungs naturally undergo physiological degeneration with
age. However, some diseases are associated with accelerated pathological aging,
such as chronic obstructive pulmonary disease (COPD). The aim of the study was
to assess the association between molecular markers of oxidative stress and
respiratory function parameters in elderly individuals and those with COPD.
METHODS : This was an experimental study involving 27 subjects, divided into
three groups: 7 healthy young adults (G1), 10 healthy elderly subjects (G2), and 10
patients with COPD (G3). All participants completed a medical questionnaire and
underwent spirometry tests with a Bronchodilator responsiveness test (BRT) using
short-acting bronchodilators. They also collected induced sputum for genetic
analysis using the RT-PCR technique.

RESULTS : The mean age of the patients was 60.42 ± 16.13 years with a male
predominance. Patients in G1 and G2 had normal spirometric data. The mRNA
expression of Peroxiredoxin 1 (PRDX1) and Glutathion ePeroxidase 1 (GPX1) was
significantly and positively correlated with the post-bronchodilator forced
expiratory volume in 1 second (FEV1)/forced vital capacity (FVC) ratio, but
negatively correlated with the degree of smoking history (pack-years). A positive
correlation was observed between the mRNA levels of PRDX1 and post-
bronchodilator FEV1 percentage change. On the other hand, mRNA levels of
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1 β (PGC1β) were
negatively correlated with post-bronchodilator FEV1 percentage change and post-
bronchodilator FEV1/FVC ratio and positively correlated with the degree of
smoking history (pack-years).

CONCLUSION : COPD is marked by an accelerated lung aging process that can
impact both respiratory and non-respiratory functions, including mitochondrial
biogenesis.

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